glpQ [2017-12-29 12:37:42]

glycerolphosphate diester phosphodiesterase, degrades wall teichoic acid during phosphate starvation

Locus: BSU02130

Isoelectric point: 9.26

Molecular weight: 32.81 kDa

Protein length: 293 aa

Gene length: 879 bp

Function: glycerol-3-phosphate utilization, degradation of wall teichoic acid during phosphate starvation

Product: glycerolphosphate diester phosphodiesterase

Essential: no

E.C.: 3.1.4.46

Synonyms: ybeD

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Utilization of cell wall components
• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of glycerol/ glycerol-3-phosphate
• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of phospholipids
• Metabolism → Additional metabolic pathways → Phosphate metabolism
• Groups of genes → Secreted proteins

Gene

Coordinates: 233,014 → 233,895

The protein

Catalyzed reaction/ biological activity

• exohydrolytic activity on wall teichoic acid, acts in concert with PhoD PubMed
• A glycerophosphodiester + H₂O = an alcohol + sn-glycerol 3-phosphate (according to Swiss-Prot)

Protein family

• glycerophosphoryl diester phosphodiesterase family (according to Swiss-Prot)

Paralogous protein(s)

• YhdW, YqiK

Structure

• 4R7O (the enzyme from Bacillus anthracis)
• 5T9B PubMed

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: glpT-glpQ
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • GlpP: antitermination, via a protein-dependent RNA switch PubMed, in GlpP regulon
  • PhoP: activation, PubMed, in PhoP regulon
  • CcpA: repression, in CcpA regulon

  Regulation

  • induced by glycerol (GlpP) PubMed

• Genes: glpQ

  Regulation

  • induced by glycerol (GlpP) PubMed

Biological materials

Mutant

• BKE02130 (ΔglpQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTTCTCCTCCTTATA, downstream forward: _UP4_TAAAAGCAAAAAACCTTGCG
• BKK02130 (ΔglpQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTTCTCCTCCTTATA, downstream forward: _UP4_TAAAAGCAAAAAACCTTGCG

References

Reviews

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Original publications

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